The objectives of this project : 1.To establish high quality grouper and small abalone semen cryopreservation techniques and industrial application. 2.To establish the MAS(marker assistant selection) for small abalone (Haliotis diversicolor), to select and develop the best small abalone for commercial aquaculture industry, to develop technique of gamete (semen) cryopreservative procedure and manipulation. 3.Developing techniques of semen cryopreservation and controlling factor during spawning to develop grouper hybridization (Epinephelus moara × Epinephelus fuscoguttatus ). 4.To provide the aquaculture industry with new techniques that were developed by the Germplasm Bank of Freshwater Fish, Fisheries Research Institute. These techniques will be promoted to the industry with following goals to enhance the competitive strength. Expected benefits: 1. To establish grouper and abalone semen cryopreservation techniques for sperm banks. The good quality cryopreserved semen can save at least 50% cost and time than fresh semen and can preserved at least 1 year which can extend the mating season of aquaculture for more than 6 months. 2.Through licensing agreements, the FRI will provide the aquaculture industry with a high-growth tilapia to commercialization. This will be beneficial for farmers to save 2-mouth farming period and to increase 5% of fillet from harvests. 3.To supply 500,000 fries of nile tilapia with the feature of high growth rate. 4.To analyze the sex-determining locus of genetically female , which will improve the selective breeding performance on the basis of male ratio. 5. Produce 400 thousands of lettered clam seeds with shell length between 0.8-1.5 cm. 6.Contact with at least two owners of aquaculture business to evaluate benefits of raising lettered clams.