Fish scale powder (FSP) with functional compounds, collagenpeptide and hydroxyapatide, which are the mainly organic composition and mineral in human bone tissue, thus the fish scale powder could be the functional resource for bone health. With this idea in mind, we examined whether FSP could be the beneficial material for bone health using lipopolysaccharide(LPS)-induced cell inflammatory in human osteoblastlike MG-63 cells as a test model. In our study demonstrated that FSP derived from Tilapia (Oreochromis mossambicus)fish scale enhanced the LPS-reduced cell viability, while the 3.86 ug/cm2 FSP showed highest cell proliferation rate being 175.38%. The cytokine ELISA kit analysis showed that FSP inhibited the expression of pro-inflammatory cytokine,including IL-6、IL-1β、TNF-α and NO, as well as, increased expression of antiinflammatorycytokine (IL-10), that represented FSP could inhibit the LPSinduced inflammation. In addition, FSP dose-dependently promoted expression of procollagen type I; increased activity of alkaline phosphatase (ALP) in osteoblast and osteocyte; promoted 2.63 times of mineralization and increased expression of osteoprotegerin under LPSinduced inflammation. Based on these findings, FSP showed promotion of bone health on increasing the activity of osteoblast and decreasing the activity of osteoclast. FSP is worth developing into a novel functional food material to serve as dietary supplement.