農業部-水產試驗所全球資訊網農業部-水產試驗所全球資訊網

本計畫全程目標擬針對我國石斑魚產業有關育種之需求: 降低寒害損失、降低疾病 損失及節省生產成本，透過開發繁殖技術並培育耐低溫石斑魚種、篩選具高成長、 高抗病種魚及透過不同種類之雜交嘗試培育出優勢的子代，達到生產耐低溫、高抗 病及成長快速之育種目標。首先，我們成功繁殖耐低溫的雲紋石斑魚，將5 尾雌魚 埋植混合雄性素藥粒，9 個月後有3 尾雌魚已性轉變為雄魚並可採集到具活力之精 子。透過人工授精共獲得135,000 粒上浮卵，其中受精率為99.67 ± 0.33%、孵化率 為99.00 ± 0.57%。受精卵卵徑為0.91 ± 0.01 mm，為圓形、透明之浮性卵。在水溫 23.8 ± 1.2℃條件下，歷經29 hr 28 min 孵化。剛孵化仔魚體全長1.77 ± 0.02 mm、肌節數21–22；孵化後第3 天仔魚體全長2.90 ± 0.02 mm，口部已開啟；孵化 後第7 天，體全長達3.44 ± 0.07 mm 時，背鰭第二硬棘及腹鰭硬棘開始發育；孵化 後第21 天，體全長達12.95 ± 0.29 mm 時，延長的硬棘開始縮短；孵化後第33 天 ，體全長達17.69 ± 0.09 mm 時，各鰭條數與成魚相同。仔稚魚培育過程餌料投餵 序列依序為牡蠣受精卵、輪蟲及橈足類。第二，我們透過分子標記輔助選種技術篩 選出2尾具有高成長及2尾具有高抗病優勢的鞍帶石斑魚親魚，可做為未來生產優質 受精卵及魚苗的親種。最後，我們透過雜交試驗可成功生產雲紋石斑魚×鞍帶石斑魚 、點帶石斑魚×鞍帶石斑魚之受精卵，並建立受精卵標準生產流程。

This project aimed to meet the breeding requirements of grouper aquaculture industry (e.g., reducing cold damage, disease losses, and saving production costs) in Taiwan. We develop breeding technology and breeding of low-temperature-tolerant grouper species, selection of highgrowth, high-disease-resistant species, and cross-breeding of different grouper species to try to breed superior offspring, to achieve the goal of producing low-temperature resistance, high disease resistance, and rapid growth. First, we successfully reported captive breeding results of kelp grouper Epinephelus moara. Implantation with an androgen mixture was induced five females sex change and obtained active sperm from three transformed males after nine months. Totally 135,000 floating eggs were obtained through artificial insemination with 99.67 ± 0.33% fertilization rate and 99.00 ± 0.57% hatching rate. Fertilized eggs with a mean diameter of 0.91 ± 0.01 mm, were spherical, transparent, and buoyant. Embryonic development lasted 29 h 28 min at 23.8 ± 1.2℃. Newly hatched larvae were 1.77 ± 0.02 mm in total length (TL) with 21-22 myomeres. Three days post-hatching (dph), the TL was 2.90 ± 0.02 mm, and the mouth opened. At seven dph, the TL was 3.44 ± 0.07 mm, and the buds of the second dorsal and pelvic fin spines had appeared. At 21 dph, the TL was 12.95 ± 0.29 mm, second dorsal and pelvic fin spines length have decreased. At 33 dph, the TL was 17.69 ± 0.09 mm, and the fin ray counts attain an adult complement. The larval rearing feeding scheme for the resulting was as follows: oyster fertilized egg, rotifers, and copepods. Second, we screened out two broodstocks of giant grouper with high growth and two advantages in disease resistance through the marker-assisted selection, which can be used as parents for future production of highquality fertilized eggs and fry. Finally, we successfully produce the fertilized eggs of kelp grouper × giant grouper (E. moara × E. lanceolatus) and orange-spotted grouper × giant grouper (E. coioides × E . lanceolatus) through hybridization experiments and establish a standard production process for fertilized eggs.