The use of Artemia nauplius on white shrimp,Litopenaeus vannamei, larvae production is important, but the price of Artemia cysts is increasing in the last decade. In this study, we want to evaluate the feasibility of replacing Artemia nauplius on white shrimp larvae production with live food that our institute conserved. First, the conserved s-type rotifers, Brachionus rotundiformis, and copepod, Apocyclops royi, were confirmed not to be infected with 10 important diseases of white shrimp by realtime PCR detection technology. In the white shrimp larvae cultivation test, the survival rate of white shrimp larvae fed with B. rotundiformis (fed at zoea stage) (60.73±13.20%) is higher than fed with Artemia nauplius (fed at mysis II stage) (41.35±4.93%). And the body length of shrimp larvae of all treatment is no significant difference. In the white shrimp postlarvae (PL2-PL9) cultivation test, the survival rate of white shrimp larvae fed with A. royi is 86.88±4.54%, and fed with Artemia nauplius is 89.55±8.04%. The survival rate is no significant difference between these two groups, and the body length gain is also the same. In summary, we can completely replace the use of Artemia nauplius on white shrimp larvae production with the use of B. rotundiformis and A. royi.