Aquaculture is one of Taiwan's important industries, with an annual value of more than 30 billion NTD. However, due to global climate change, extreme cold weather in winter, heat waves in summer and heavy rainfall in a short period of time, it has caused significant losses to aquaculture fisheries. The project will manipulate rising and falling temperature modes, and explore the physiological state of grouper, milkfish and white shrimp in temperature stress, and evaluate through physiological and biochemical indicators, molecular markers, nutrient feed formula, facility improvment, to increase the environmental tolerance of the aquaculture species. The final research results will develop a variety of economic aquaculture species resistant to extreme weather and adversity, as well as the optimal aquaculture management model, providing high survival rate and low disaster losses to maintain food production safety and enhance the industrial resilience of the aquaculture industry in the face of climate change scenarios.