AFLP technique, developed by Vos et al. (1995), is based on the selective PCR amplifiedof restriction fragments from a total of genomic DNA. To research and identifynumbers of AFLP markers, 225 primers combinations, base on the restriction enzymeEcoRI and HindⅢ with two base pair extensions, were tested to generate AFLP pattern.The markers are suitable for use in gene pool and trait loci by using the inter- and intraspecies.Analysis of genetic variations among strains of fish indicated that AFLP shouldalso be a valuable tool for genetic resource and population analysis in fish.