農業部-水產試驗所全球資訊網農業部-水產試驗所全球資訊網

由於目前花鰹屬仔魚依據外部形態只能鑑定到屬的層級，因此本研究先利用mtDNA區分出花鰹屬內的兩種仔魚 ( 平花鰹及圓花鰹 ) 再根據結果回推平花鰹與圓花鰹在外部形態上的差異。結果顯示，在平花鰹 ( Auxis thazard ) 的眼部後方有一黑色素胞存在，而圓花鰹 ( Auxis rochei ) 則否。此外，利用PCR-RFLP方式，以BglΠ與Bpu10I兩組限制酶也可快速的將圓花鰹、平花鰹確實區分：(1) 以限制酶BglΠ作用花鰹屬仔魚之CO I基因共1946 bp，平花鰹可得約1676 bp、270 bp兩不同長度之片段，而圓花鰹無反應。(2) 以Bpu10I作用，平花鰹無反應，而圓花鰹可得約1183 bp、551 bp、212 bp三長度不同之片段。

Because the larvae of Auxis identification is difficult depending on morphological characters, the present study is based on mitochondrial CO I sequencing to distinguish A. rochei and A. thazard. Behinding the eyes of A. thazard exist a melanophore as a result, and it is a lack at A. rochei. Moreover, we using polymerase chain reaction – restriction fragment length polymorphism ( PCR-RFLP ) technology with two restriction enzymes contains BglII and Bpu10I to identify A. rochei and A. thazard. (1) The restriction enzyme BglII cleaved the 1946 bp fragment to 1676 bp and 270 bp in the larvae of A. thazard, and there was no any cutting sites in A. rochei. (2) The restriction enzyme Bpu10I could cleave the 1946 bp fragment to 1183 bp, 551 bp, and 212 bp in the larvae of A. rochei, but there was no cutting sites in A. thazard.