農業部-水產試驗所全球資訊網農業部-水產試驗所全球資訊網

因罹患神經性壞死病毒(NNV)及虹彩病毒(IRV)而造成養殖石斑魚大規模死亡，本計畫利用目前已經開發之病毒性疫苗，來建立一基準之石斑魚疫苗評估與測試之技術，本計畫目的利用目前已經開發之石斑魚IRV疫苗，使用於石斑魚苗後，觀察石斑魚抵抗IRV感染之效果。根據試驗結果顯示，試驗結果顯示，疫苗注射對於4-6公分石斑魚苗之安全劑量為0.01-0.2 ml/fish。而實驗室攻毒結果，經IRV疫苗處理石斑魚苗2、4、與6週後，對於IRV感染之抵抗能力，較未經疫苗處理之試驗魚強(p<0.01)，活存率約可增加50％。在抗體中和力價方面，於IRV疫苗注射後二周達到高點，一直持續八週以上。

The nervous necrosis virus (NNV) and iridovirus (IRV) have been attributed to a measurable mass mortality of the grouper. This study aims to develop the techniques to improve the effect of vaccine to induce protection of grouper against virus infection. The results showed that the safety injection IRV vaccine was 0.01 to 0.2 ml/fish to the grouper fry (4-6 cm). In the challenge test, the survival rate of the vaccine groups of fish challenged with injection of IRV diluted solution was higher (p<0.01) than control group fish over 50% after IRV vaccine treatments for 2, 4 and 6 weeks. In the neutralization test of test fish serum collected in different period after injection of IRV vaccine showed that the highest title of grouper serum was appeared at 2 weeks after vaccination and last over 8 weeks.