The present work was devoted to investigation of the fructooligosaccharide (FOS) and levan forming activity of marine bacterial isolates. Among the five strains of isolates from marine tilapia cultured pond, the amount of kestose and levan produced by the strain T1 was the highest at 24 hr (1.69 mg/mL) and 72hr (145.56 mg/mL) (28℃) in medium containing 8% sucrose, respectively. The strain was identified as Bacillus licheniformis, and named as FRI-MY-55. The FOS and levan production by Bacillus licheniformis FRI-MY-55 depended on the concentration of sucrose used. The maximum levan productivity (457.69 mg/mL) was obtained on the medium containing 400 mg/mL sucrose. After cell separation the culture liquid containing 200 mg/mL sucrose was treated with ethanol to precipitate levan together with extracellular levansucrase. Levan- levansucrase sediment be used as biocatalyst for FOS and levan production. The kestose content reached maxium at 12 hr (26.90 mg/mL), then decreased gradually with the prolonged reaction time, while the levan content continued to increase during all 48 hr of incubation, at 48 hr, the amount of levan of 1162.08 mg/mL was obtained. The levan product consisted of three fractions with different molecular masses (5 KDa, 11.6 KDa and＞668 KDa). The temperature affected the fructosyltransferase ability production by Bacillus licheniformis FRI-MY-55. At 50℃, the highest ability was observed, and the level of kestose reached at 10.561 mg/m