Nervous necrosis virus ( NNV ) infective grouper larvae that causes high mortality, resulting in serious economic losses in aquaculture industry. The purpose of this project will apply proteomic method to analyze viral protein and virus-host interaction. It will provide new insights into understanding of action mechanism of nervous necrosis virus. The first year of this project used grouper cell line to build proteomic technology-protein extraction, two-dimensional gel electrophoresis, in-gel digestion, MS sequencing and protein database analysis. We have established techniques of proteome and cell culture for future investigation.