農業部-水產試驗所全球資訊網農業部-水產試驗所全球資訊網

本計畫的總目標為完成至少二種水產檢疫診斷用試劑的研發，目前規劃以造成海水魚及淡水魚養殖主要病害的Aeromonas hydrophila及Vibrio spp.為試劑開發目標。重要工作項目包括將目標菌株與淡水環境常在菌30株、海水環境常在菌30株、台灣常見水產病原菌20株（包含Aeromonas hydrophila、Aeromonas salmonicida、A e r o m o n a s s o b r i a、E d w a r d s i e l l a t a r d a、E n t e r o c o c c u e f a e c a l i s、Enterococcue faecium、Flavobacterium columnaris、Lactococcus garvieae、Listonella anguillarum、Nocardia asteroides、Photobacterium damselae subsp. damselae、Photobacterium damselae subsp. piscicida、Pseudomonas
aeruginosa、Pseudomonas anguilliseptica、Staphylococcus epidermidis、Streptococcus iniae、Vibrio alginolyticus、Vibrio harveyi、Vibrio parahaemolyticus及Vibrio vulnificus）同步進行各菌株間形態、色素、運動模式、氧氣需求、適溫、適鹽、藥物耐受性、酵素活性、抗凝集性、APITM、MICROBACTTM生化試驗、16S rDNA、16S-23S rDNA序列之分析比對、從各種層次探討目標菌株之特異性。本年度的預期效益為完成實驗室級Lactococcus garvieae 檢測套組原型。

The final aim of this project is to develop at least two detecting kits to aquaculture pathogens. Aeromonas hydrophila and Vibrio spp. which cause major diseases to freshwater and sea water aquaculture,respectively, are the most possible targets. The main works are to analyze the differences of morphology, pigment, motility, oxygen demand, temperature tolerance, salinity tolerance, chemical tolerance, enzyme activities, anti-agglutination patterns, API ＆ MICROBACT biochemical assays, 16S rDNA, 16S-23S rDNA alignment among target bacterium and twenty strains of common aquaculture pathgogens（including Aeromonas - 14 - TS2011021016023029320100/02/10 16:02:30hydrophila、Aeromonas salmonicida、Aeromonas sobria、Edwardsiella tarda、Enterococcue faecalis、Enterococcue faecium、Flavobacterium columnaris、Lactococcus garvieae、Listonella anguillarum、Nocardia asteroides、Photobacterium damselae subsp. damselae、Photobacterium damselae subsp.piscicida、Pseudomonas aeruginosa、Pseudomonas anguilliseptica、 Staphylococcus epidermidis、Streptococcus iniae、Vibrio alginolyticus、Vibrio harveyi、Vibrio parahaemolyticus及Vibrio vulnificus） in Taiwan.The expect results of this year is to develop a prototype of Lactococcus garvieae detecting kit which can be used for research purpose.