1、The cultured copepod, enriched Artemia nauplii, and Artemia nauplii were fed to make comparision of the growth and survival of newborn Hippocampus spinosissimus. After a 28-day rearing period, the survival of Hippocampus spinosissimus juveniles fed copepod was 70.67±6.11﹪,and was significantly higher than those fed enriched Artemia nauplii（12.00±6.93﹪）,Artemia nauplii（4.00±4.00）（p<0.001）. The survival of juveniles fed enriched Artemia and unenriched Artemia was not significantly difference（p>0.05）.
2、The result showed that, better survival rate and growth were obtained under feeding with the nauplii of Artemia nauplii at 2 and 4/ml of feeding on 10 dph (days posthatch) larvae. About the delay of feeding, better survival rate and growth would be obtained under feeding within 2 dph. The survival rate was better in which were raised at 24, 29, 34 and 39psu. About the control of environment temperature, better survival rate was obtained in which were raised during the water temperature of 24-30℃, and better growth was obtained at 30℃ .
3、In the freshwater ornamental fish species, the freshwater stingray are expensive aquarium pets, but ornamental fish breeders generally considered difficult to breed freshwater stingray. This study was to explore that different sex ratio of male and female rays will not affect reproductive outcomes, the size of new born rays and the female parent rays was positively correlated.
4、Establish artificial propagation techniques for Blue Tang (Paracanthurus hepatus), transferring to the fishery as a reference for rear other species. Wild-caught broodstock were spawned naturally in captivity, without the use of hormones or other treatments, from May to October 2010. The eggs of Blue Tang amyo buoyant and semi-transparent. The egg diameter range from 0.67 ± 0.04 mm . The eggs contain a single oil globule having a diameter of 0.15 ± 0.01 mm. Incubation period was approximately 18 h at the water temperature of 27.6 - 29.1 ℃ and salinities at 32 psu. The newly hatched larva was 2.165 ± 0.36 mm in length. On 3 DPH after hatching (2.49 ± 0.054 mm), Yolk using exhausted, the mouth can begin to prey on the formation of 70~80 μm ciliate. On 7 DPH, rotifers was supplied as the feeds, then copepod was supplied as the feeds on the 12 DPH.
5、The results showed these adhesive demersal eggs are white to yellow and ellipsoidal, and were about 1.22±0.05 mm（Mean±SD） in total length and 0.63±0.02 mm in width. The yolks were about 0.73±0.05 mm long and contained several oil droplets with diameters ranging 0.10± 0.05 mm mm and the newly hatched larvae were about 2.73 ± 0.30 mm in length. Parental care behaviors for the ovum occurred after spawning to hatching. It indicates that parental behavior is mainly carried out by males. Incubation period was approximately 100 h at the water temperature of 26±0.5℃.The biological feedings for Molucca Damselfish at initial larvae stage was ciliate, rotifer and copepod. Microalgae was added to stabilize water quality and to nourish rotifers. The supply of feedings should be applied at the same time.
6、In this study, we obtained 57 cases collected from 11 ornamental fish farms（52 cases from freshwater ornamental fish and 5 cases from seawater ornamental fish）in distinct regions of Taiwan, 2010. Gross external and internal lesions were recorded. Samples were identified using light microscope examination, microbiological examination and polymerase chain reaction (PCR). In examination result, the bacterial disease were 70.2％ (n=40) in ornamental fish included Aeromonas sorbria (n=11), Mycobacterium sp. (n=11) and Aermonas hydrophila (n=4) etc. The parasitic disease were 43.9％ (n=25) in ornamental fish included Ichthyophthirus sp. and Cryptocaryon sp. (n=5), , Dactylogyrus sp. (n=3), Tetrahymena sp. (n=3), Camallanus sp. (n=3) and Epistylis (n=3) etc. The viral disease in ornamental fish included Koi herpesvirus infections (n=3) and Iridovirus infections (n=3). Saprolegniasis (n=2) also have been observed in Koi (Cyprinus carpio). In addition, the PCR technique was established to act as a fast and accurate diagnostic method for monitoring Aeromonas hydrophila, Vibrio vulnificus and Koi herpesvirus in ornamental fish.
7、In varied anemonefish species, the optimal temperature and salinity in packaging and transportation environment to Amphiprion percula, A. sandarcinos and A. nigripes were 20, 22, 22℃ and 28, 30, 30 ‰, respectively. In varied damselfish species, the optimal temperature and salinity in packaging and transportation environment to Chrysiptera cyanea, C. parasema and Chromis viridis were 18, 20, 20℃ and 26, 30, 30 ‰, respectively. In varied seahorse species, the optimal temperature and salinity in packaging and transportation environment to Hippocampus kuda, H. trimaculatus and H. histrix were 22, 22, 24℃ and 30 ‰, respectively. The minimum packing unit weight of Anemonefish, damselfish and Hippocampus of the water demand were 45-65,35-55 and 85-105 ml / g whole body weight, and varied species of coral shrimp was 75-115 ml / g whole body weight, while packing environment of salinity and temperature should be controlled at 30-32 ‰ and 22-24 ℃.