農業部-水產試驗所全球資訊網農業部-水產試驗所全球資訊網

本研究從野生與池中培育之草蝦中篩選出不帶白點病毒（WSSV）、桃拉病毒（TSV）與傳染性皮下及造血組織壞死病毒（IHHNV）的SPF之草蝦種蝦，進行SPF蝦苗繁殖；先後購入由東港沿近海捕獲的性成熟雌草蝦6尾，經PCR檢測後確定有4尾為SPF種蝦，同時池中培育的成蝦經篩選與PCR檢測後，共選出50對進行單眼柄切除人工催熟；4尾SPF的野生種蝦生產之蝦苗經培育與檢測後，篩選出共20萬尾進行選育種用，池中培育的SPF種蝦生產之蝦苗經培育與檢測後，亦篩選出共20萬尾進行選育種用，養成過程中進行分段篩選。
蝦苗進行攻擊試驗，篩選對病毒耐受性高的蝦苗進行養殖，將兩來源種蝦生產培育之蝦苗依種蝦個別為依據分批抽樣進行攻擊試驗，試驗分別進行WSSV與低溫耐受性之比較，WSSV耐受性攻擊試驗最高活存率為36.3﹪，最低活存率為18.3﹪；低溫耐受性試驗最高活存率為21.6﹪，最低活存率為8.1﹪。

The specific pathogen free (SPF) broodstock of Penaeus monodon with White Spot Syndrome Virus (WSSV)、Taura syndrome virus（TSV） and Infectious Hypodermal and Hematopoietic Necrosis Virus (IHHNV) were sieved from wild species for breeding SPF larvae. The six sexual mature female P. monodon were wild-caught broodstock of Tung Kang, and four of the six were SPF following PCR examination. Moreover, after sieving and PCR examination, 50 pairs of adult P. monodon with ablation eyestalk were conducted from the cultivating ponds. The amount of 200,000 larvae from the four wild species P. monodon were sieved and examined for breeding, and the same procedure was carried in the cultivated ones. Following the developing stages, the larvae were sieved during cultivation.
The larvae were challenged for sieving high tolerance to virus infection for further cultivating. The two resources of larvae were randomly sampled for challenging tests according to each individual brood stock. The challenging test consisted of WSSV infection and tolerance to hypothermal exposure. The highest survival rate of WSSV challenging test was 36.3%, and the lowest one was 18.3%; the highest survival rate under hypothermal exposure was 21.6%, and the lowest one was 8.1%.
Keywords：White Spot Syndrome Virus（ WSSV）；Penaeus monodon；Specific Pathogen Free（SPF）