The objective is to establish the biosecure culture system of Macrobrachium rosenbergii and to establish its fast growth SPF broodstock. From the results of 10 months culture of four stocks of M. rosenbergii, growth were fastest in North-Ligang Jiouru, followed by Jiouru, South-Ligang and, Yenpu; average body weight of each group were 45.5 g、41.7 g、39.4 g、34.2 g, respectively. However, South-Ligang had highest survival rates (74.7%), followed by North-Ligang (63.8%) Yenpu (55.4%) and Jiouru (53.0%). Larvae were reared in 10 ton FRP tanks without changing water, survival rate reached to 55.0% with average of 40.8%.