The Grouper is better delicious and high economic value. It is one of the important species of Marine fish farming for the current. The annual output reaches above 12,000 metric tons, among the main of Epinephelus coioides. At present raised still use the trash fish feeding group for many, because the trash fish was unstable to originate on the fresh and health, got down the cultivation result, to strive for the industrial promotion and the product safety and health, planned to develop the fattening diets of finishing period to raise the cultivation benefit. The dietary contents 45% protein and 12% fat to have better growth of groupers over 400g. Preliminary estimated the optimal growth energy is 1538 Kjoule / 100g diet, protein and energy ratio is 29.26 mg /Kjoule. The growth, Feed efficiency, Condition factor and Vicera-somatic index not affected with used soy oil substitutes for 60~70% fish oil.