農業部-水產試驗所全球資訊網農業部-水產試驗所全球資訊網

本研究利用臭氧洗卵技術降低或阻斷所有病原的垂直感染。本研究並利用SPF育苗技術，包括在SPF養殖池培養微藻、輪虫、橈腳類豐年蝦等餌料生物，以完全阻斷所有病原的水平感染途徑。本研究並建立PCR技術針對神經壞死病毒(NNV)和虹彩病毒( IV)進行監控，藉以提升魚苗的存活率，並生產SPF鞍帶石斑魚苗。本研究在整個繁養殖過程都不使用任何藥物的情形下，沒有任何疾病發生，平均育苗率3.37%、平均中間育成率89.3%。將4吋的魚苗360尾在50㎡圓池養殖10個月後，均重2,308 g、活存率98.1%、產量16.3 kg/㎡。
關鍵詞：生物防疫;無特定病毒;鞍帶石斑;神經壞死病毒;虹彩病毒;

The objective is to establish the biosecure culture system of grouper (Epinephelus coioides). The PCR techniques were developed and used to screen the existence of NNV (nervous necrosis virus) and IV (Iridovirus )。Viruses on the surface of grouper embryos were killed using ozone. The survival rates were more than 3% from hatching to one inch body length, 89.3% from one inch to 2 inches and 98.1% from 4 inch to 2.3 kg in 10 months without using any chemicals.