農業部-水產試驗所全球資訊網農業部-水產試驗所全球資訊網

35~40℃低溫乾燥後進行臼磨機進行磨粉，Rhodobacter sphaeroides、Bacillus subtilis、Enterococcus faecalis、與Micrococcus luteus等4株細菌復活率平均為87~92%，Lactobacillus acidophilus 較低，平均為55~60%；微粒包覆活菌進行噴霧乾燥機乾燥，噴霧乾燥機噴出口溫度較高設定為105 ℃，乾燥後進行臼磨機進行磨粉並進行細菌復活性試驗，結果顯示： Bacillus subtilis、Enterococcus faecalis、Micrococcus luteus等3株應用細菌復活性較好，平均復活率為80~88%，Rhodobacter sphaeroides與Lactobacillus acidophilus復活率為50~60%。微粒包覆疫苗與olive oil佐劑配合微粒包覆應用微生物，進行試驗魚免疫餵食試驗，結果顯示，m＊-vaccine with m＊-Bacillus sustilis、m＊-vaccine with m＊-Rhodobacter sphaeroides、m＊-vaccine with m＊-Enterococcus faecalis、m＊-vaccine with m＊-Lactobacillus acidophilus、及m＊-vaccine with m＊-Micrococcus luteus等5組試驗組RPS都在87%以上，都可以得到不錯的保護力
關鍵詞：
光合菌、枯草菌、乳酸桿菌、腸球菌、微球菌、巨噬細胞、吞噬作用

The experimental tilapia(Oreochromis nilotica)were fed with dietary eel diet containing different microcapsuled-vaccine(anti-Strep. iniae) and dry microcapsuled-probiotics powder for 14 days(28-30℃), and challenged with Strep. iniae. It was showed good protect effect in five experimental group inclucing m＊-vaccine with m＊-Bacillus sustilis、m＊-vaccine with m＊-Rhodobacter sphaeroides、m＊-vaccine with m＊-Enterococcus faecalis、m＊-vaccine with m＊-Lactobacillus acidophilus、and m＊-vaccine with m＊-Micrococcus luteus.