Mycosporine-like amino acids (MAAs) are a family of intracellular
UVabsorbing compounds involved in the protection of marine organisms
against solar UV radiation. In this study, we focus on the safety and
functional evaluation of MAAs extracted from Gracilaria. The safety
evaluations of MAAs were determined in the Ames test, which was conducted
by the plate incorporation method using five strains of Samonella
typhimurium. The functional evaluations of MAAs were determined in the
anti-aging actions of MAAs on UV-induced aging in skin fibroblasts. The
fibroblasts were pretreated with MAAs. After UVB irradiation, the levels
of aging were determined by measuring MTT assay, Intracellular ROS
content and the expression of matrix metalloproteinases. Finally, we
estimate the condition of preparation, extract process and equipments for
20 kg Gracilaria (dry base) per batch.