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乳酸菌發酵紅藻之產品開發(2/2)

  • 日期:107-02-20
  • 計畫編號:107農科-9.4.2-水-A1(2)
  • 年度:2018
  • 領域:漁業科技研發
  • 主持人:蔡慧君
  • 研究人員:高淑雲、易琮凱

 本計畫以106年所建立之自篩乳酸菌F1(Pediococcus pentosaceus)發酵海木耳( Sarcodia montagneana)之較適發酵製程,製備發酵產物,並分析海發酵前後之總酚 、水溶性蛋白、胜肽與糖類變化,總糖與還原糖濃度由69.70±0.38mg/g與 3.01±0.06mg/g下降為43.78±0.18 mg/g與2.26±0.04mg/g,推測應是乳酸菌利用所致 ,而總酚、水溶性蛋白與胜肽濃度則由2.47±0.01mg/g、51.26±2.6μg/mg、 49.75±1.93μg/mg增加至3.71±0.02mg/g、65.58±2.06μg/mg、 61.27±1.89μg/mg,觀察發酵後成分的變化,總酚增加1.50倍,水溶性蛋白增加 1.28倍,胜肽則為1.23倍,其中以總酚提升比例較高。

 探討海木耳熱水萃取物、發酵產物,對毛囊細胞(SV-40)增殖作用。試驗結果,市 售藥物米諾地爾(Minoxidil)有最高的增殖作用,海木耳發酵產物在濃度達1mg/mL時 ,其增殖率最高,相較於熱水萃取物,具有顯著的增殖表現。再者,對毛囊細胞5- α還原酶抑制作用。結果顯示,市售藥物度他雄胺(Dutasteride)有最高的抑制效果 ,而發酵產物也具有很好的抑制效果,且呈現劑量依存性。

 海木耳乳酸菌發酵產物軟質凍飲試製及官能品評,首先萃取海木耳之色素,經由本 研究建立之萃取技術所得色素,呈現紫紅色澤,以此進行複合配方的調製,在官能 品評結果,於外觀、顏色、風味、軟硬度及整體接受度,均可達6分以上,顯示官能 接受度高。以海木耳乳酸菌發酵產物為主,試製頭皮外用劑,包括原液劑型及微乳 化劑型,並進行貯存安定性評估,於高溫加速試驗(50℃,14天)、循環變溫試驗 (50℃←→室溫←→4℃,14天),其結果顯示,上述2者皆具有良好之貯存安定性。

研究報告摘要(英)


 The plant prepares the fermentation product by the suitable fermentation process of the self-screening lactic acid bacteria F1 (Pediococcus pentosaceus) fermented red algae (Sarcodia montagneana) established in 2017, and analyzes the total phenol and water-soluble protein before and after sea fermentation. The peptide and saccharide change, the total sugar and reducing sugar concentration decreased from 69.70±0.38mg/g and 3.01±0.06mg/g to 43.78±0.18mg/g and 2.26±0.04 mg/g, which is supposed to be caused by the utilization of lactic acid bacteria. The concentration of total phenol, water-soluble protein and peptide increased from 2.47±0.01mg/g, 51.26±2.61 μg/mg, 49.75±1.93μg/mg to 3.71±0.02mg/g, 65.58±2.06μg/mg, 61.27±1.89μg/ mg, observed changes in the composition of the fermentation, total phenol increased by 1.50 times, water-soluble protein increased by 1.28 times, peptides were 1.23 times, of which the proportion of total phenol increased.

 To explore the proliferation of hair follicle cells (SV-40) by extracting hot water extracts from Sarcodia montagneana  and fermentation products . The results showed that Minoxidil, the main ingredient of the commercially available drug xinfaling, had the highest proliferative effect. The fermentation at the concentration of 1mg/mL was the highest. Hot water extract with significant proliferative performance. Furthermore, it inhibits the hair follicle cell 5-alpha reductase. The results showed that Dutasteride, the main component of the commercially available drug, had the highest inhibitory effect, while the fermentation product also had a high inhibitory effect and showed dose dependence.

 The trial preparation and functional evaluation of the soft drink of lactic acid bacteria fermentation of haimuer, firstly extracting the pigment of auricularia auricula, and the pigment obtained by the extraction technique established in this study, showing a purple-red color, thereby preparing the composite formula, in the functional evaluation result, in appearance, color, flavor, hardness and overall acceptance can reach 6 or more points, indicating high functional acceptability. The main products of the scalp lactic acid bacteria were prepared, including the squeezing external preparations, including the original liquid dosage form and the micro emulsifier type, and the storage stability evaluation was carried out in the high temperature accelerated test (50℃, 14 days) and the cyclic temperature change test (50℃←→room temperature←→4℃, 14 days), the results show that both of them have high storage stability.