The objectives of the project are to establish the SPF broodstock of Litopenaeus vannamei and to improve high growth and high reproductive performance through selective breeding. The results are following: 1.Introduce of broodstock: Four batches of broodstock were introduced and kept in Tungkang Biotechnology Research Center, Taiwan Fisheries Research Institute. 2. Diseases diagnose: broodstock were screened with WSSV, TSV, YHV/GAV, IMNV, IHHNV and EMS pathogen, Vibrio parahaemolyticus using qPCR . 3. Selection for high reproductive performance: the number of spawn were highly variable within 107 females used in this study, in which, 2 no spawn at all; 12 spawn more than 9 times, which spawn 111 times in total and represents 22% of the grand total of 499 times. 4.Selection for high growth: This study using the offspring of three batches with high reproductive performance to go through 2-step high growth selection program. The selective intensity for the first and the second step of selection were 17-18% and 25-33%. 5.Compared growth performance with different selective generation: The growth rate(g/week), FCR, survival rate(%) and yield(kg/m 2 ) were all not significantly different of white shrimp between local breeding(1-2 generation) and F1 of imported broodstock at density 150/m2 and 200/m2 for 16 weeks. It showed that the seedlings with 1-2 generations of local breeding had the same competitive ability as the F1 of imported shrimp.