In this study, we added 8% highly oxdized soybean oil, which had 202.64 mmol/kg peroxide and 156.63 nmol/g malondialdehyde (MDA), to experimental diets with differente final contents of vitam C and E (C50/E50, C500/E50, C50/E500 and C500/E500), and fed tilapia for 8 weeks. The results showed that oxdized soybean oil had adverse effect on growth performance, and there were no positive effects on increasing vitamin C or E content. In contrast, high level of vitam C or E can reduce fillet a value of color space. Moreover, Vitamin E also can increae CIE L* value. The result indicated both vitamin C and E can promote fillet coloration. After 7 days storage, both vitamins can significantly inhibit MDA generation, and significantly redeuced CIE a* value. Texture profile analysis showed that vitamin C or E had no significant effects on fillet texture. In the aspect of health status, 500 mg/kg vitamin C combined with 500 IU/kg vitamin E can reduse plasma alanine transaminase activity, alleviating negative effect caused by oxidized oil. 0, 5, 10 and 20% corn gluten meal (CGM) were added to diet and fed tilapia for 4 weeks, the results showed there was negative correlation between CGM content and CIE a* value. In addition, CIE b* value did not significantly increase after 4 weeks feeding.