The goal of this project are (1) Investigation and establishment of red tilapia strains. (2) Mating the red tilapia with the supermale tilapia and analysis the male ratio of offspring. Seven restriction enzymes were used to analyze the fragment polymorphisms of the three red tilapia strains from FRC (R1, R2 and R3) and the fish imported from Singapore (RS), and showed that R1, R2 and RS are similar with Oreochromis mossambicus. There are two types of R3 strain, which are close to O. niloticus and O. aureus. In addition, in the analysis of the male ratio of the offspring, the R1 strain has the highest male rate of offspring, with a male rate as high as 100%, followed by the R3 strain with a male rate of 97.6%, and the R2 strain has a lower male rate. 71.1%.
The aim of this research is cross with TFS strain to improve the salt-tolerant of red tilapia. We built TFS sea tilapia strains, but not as good as the market size and growth of the Nile tilapias. Therefore, to improve the seawater adaptability of tilapia, we bred TFS strain and the red TsRn strain from the Nile system, then established forward and diallel crossing F1, inbreeding F2 and backcross generation BC1F2. However, their self-bred offspring have completely different performances. The backcross test confirmed that the salt-tolerant traits mainly
come from TFS strain. There are at least 5 sets of molecular markers that can distinguish the growth rate this year. In addition, the whole genome sequencing is still in progress. The read N50 of the raw sequence is 23,492 bp, and assembly are 10,285 contigs, of which the Contig N50 are 27,500 bp, It means the Highaccuracy genome sequences should be available.