This year (2021), we successfully cryopreserved the semen of 5 potential giant groupers (Epinephelus lanceolatus) and tiger groupers (E. fuscoguttatus). The sperm motility was greater than 75% and the preserved volume was 15-16.5 ml and 3-4.5 ml, respectively. All semen were NNV free. Second, we used gene expression, simple sequence repeat (SSR), and single-nucleotide polymorphism (SNP) to analyze the high growth and disease resistance biomarkers of giant grouper and tiger grouper broodstocks. The results showed that ample high growth gene markers were found in nine giant groups and five tiger groups. The high disease resistance gene markers were found in seven giant groupers and three tiger groupers. These dominant broodstock are helpful for the subsequent production of dominant offspring and hybrids. Finally, the captive breeding of yellow grouper (E. awoara) which with high market potential and low-temperature resistance was developed in the present project. The year-round gonad development was investigated. The spawning season was from February to April and the reproductive behavior occurred in 14:00-18:00. We observed two males chasing one female, and finally swam to the water surface and release sperm and eggs at the same time. Embryonic development and larvae hatched lasted 39 h 01 min at 21.5 ± 0.5℃. The goal of the project will preserve the sperm of advantage grouper species, establish a database of superiority broodstock, and develop emerging grouper propagation technology to enhance the well-being of fish farmers and promote the industry's sustainable development.