農業部-水產試驗所全球資訊網農業部-水產試驗所全球資訊網

自台灣淡水或海水區域挑選75個紅色菌落進行分析，發現可產還原蝦紅素之菌株，經傳統微生物生化與16S rRNA鑑定為Paracoccus sp.，編號為AS-14。以Marine broth (MB)進行培養，結果顯示較適培養條件為鹽濃度3%下，於25℃ 100 rpm震盪培養3天可得最高還原蝦紅素含量(0.02~0.05 mg/mL)。MB中添加0.25% sucrose可作為AS-14菌株之最適碳源，使得還原蝦紅素產量可達0.20 mg/L。另一方面，額外添加氮源反而造成還原蝦紅素含量下降。但是添加0~1.0%之消泡劑並不會影響AS-14生長與還原蝦紅素之產量。發酵槽進行3 L發酵，在100 rpm轉速下，通氣量0.7 VVM可達最高菌量數(9.8 g)，還原蝦紅素之產量則在1.0 VVM下達到最高(589.1 μg/g)，隨後則開始減少。以Tryptic soy broth (TSB)進行培養，發現添加0.25%氯化鎂進行培養所得還原蝦紅素之產量可達0.50 mg/L。經NTG突變結果突變株AS-14ML32還原蝦紅素產量可提升至6倍。

Isolation of 75 red colonies from fresh water or sea area of Taiwan were analyzed and found to restore an astaxanthin production of strain named as Paracoccus sp. No. AS-14 by according to traditional biochemical and 16S rRNA identifications. The optimal culture conditions of this strain could produce the highest astaxanthin content (0.02~0.05 mg/L) when incubated in Marine broth (MB) containing 3% salt concentration at 25 oC, 100 rpm for 3 days. In MB containing 0.25% sucrose was the suitable carbon source, however, the astaxanthin yield were considerably reduced when additional nitrogen sources were added. 0 ~ 1.0% concentration of defoaming agent did not influence the growth and astaxanthin content of AS-14. When 3 L fermentation was carried out in a fermentor at 100 rpm, a maximum amount of cell mass (9.8 g) was harvested at the 0.7 VVM aeration rate. When aeration rate up to 1.0 VVM, the yield showed the highest astaxanthin content (589.1 μg/g). When AS-14 incubated in TSB containing 0.25% MgCl2 could produce 0.50 mg/L astaxanthin. A mutant AS-14ML32 after mutagenesis with NTG could increase astaxanthin production to 6-fold higher than AS-14.