1.A novel culture system was designed for save energy and space in this project. This novel culture system could be used for commercial ornamental fish mass production.The important experimental item contained: (1)The experiment for the capability of high efficiency solid and solution separation unit and micro-particle filtration. (2)The experiment for the capability of photoelectrocatalytic oxidation. (3)The experiment for the capability of novel culture system. (4)The experimental for one species of ornamental fish mass production.The survival of ornamental fish mass production will increase. 2. Establish artificial propagation techniques for Paracanthurus hepatus,transferring to the fishery as a reference for rear other species. Establish the techniques on rearing parent Paracanthurus hepatus to achieve the goal of natural mating and spawning and rearing parent Paracanthurus hepatus for more than 100 individuals.Establish the techniques for larval rearing of Paracanthurus hepatus and understand the requirement of feedings, water quality and microalgae as a reference for massive larval rearing.3.Marine ornamental shrimps are a group of living creatures with tiny figure, bright colors, unique behaviors, and high prices in ornamental market. Some of those has bred successfully under laboratory methods and fully cultivated. Due to the restriction of space and equipments of experiment place, only basic biology researches of breeding and cultivation were held. But these results have cumulative scientific data of breeding and cultivation. This project will apply former research results on actual rearing methods of adult shrimps, and make adult shrimps produce larvae continuously under fine environment. At the same time, larvae will grow stably under good condition and bait control, and also, setting up the standard processes to achieve the purpose of commercial production. 4.The study will build technique of the water treatment for closed type transportation of live fish. The carbon dioxide transfer to calcium hydrogen carbonate to release the fish stress by using ceramic ball which is producing from oyster shell. The ball can absorb the ammonia from fish metabolism to protect the fish to poison, and the fish survival rate isincreasing. The prescription and size of the ball is adjusted with the different fish which transportation by entrepreneur. The transportation time and the density of fish in the package increasing by the ball. Thus,the aim of decreasing cost of transportation can be achieved. The work items of the study are producing the ceramic ball, to analyze the ball effects on the treated water, to simulate the transportation of live fish, and to evaluate the cost of the ball and transportation. The prospect benefits are developed the ceramic ball which is valuable commercial use. It is obviously to improve the fish stress and increasing the live fish transportation time.5.Study of the packaging and transportation techniques to current ornamental aquarium trade. To survey and analyze the key factors, including the main effects, technology can improve the correlation with the lack of management, and the main trade partners for the local production with market, development potential species. Try to establishment of proper packaging techniques. By water quality control, stabilization and/or anesthetic treatment, reducing transportation costs, risk for mortality and improved packaging materials, providing reference for the local industry standard packaging operations processes. To develop the techniques which practical application of intensive packaging and long-distance transportation. By reducing the shipment costs and improve survival ration, to ensure the local products competitive in the
international market