1. Develop the novel techniques of stocking, single packaging and long term transportation to ornamental fish, including the 4 species pleco (Loricariidae), 6 species Africa rift lake cichlid, 4 species tiny Characidae, 4 species Corydoras and 4 species reef fish, respectively. To develop the technology of single packing, absorbability materials, multiple layer stack and temperature control, not only rise the survivorship 15％ at the least, but also enhance 20％ capacity and keep the DA7 under 2％.
2. We continue the work of the last-year project which proposed development of commercial product for ornamental fish transported in closed with the use of water conditioner. The composition and dosage of the water conditioner were be optimized by fish transport performance and established TFRIN-B formula. Simultaneously analysis the different transport temperature and different payloads density with simulated live transportation of fishes for 72 hours. After the testing, we prepare 100 sets of TFRIN B for some industry and compare with commercial available product on the practical of operation. Live fish transportations of domestic or international air line by using TFRIN B were tested also. All of the fish was 100％ survival on arrival day1. Suitable for TFRIN B formulation into a slow-release tablet was validated with safety and stability for six months. No significant effects on Survival rate at or water parameter after live fish transport. With the observed results, we are able to conclude that the TFRIN B is efficient for live fish transport, high survival rate and helping maintain water quality possibly by lowering metabolic wastes after fish shipment and has shown commercial potential.
3. The optimum rearing conditions for dancing shrimp Rhynchocinetes durbanensis larvae were: feeding Artemia nauplii 1 ind./ml, fed on the first day after hatching under 24-27℃. But Cinetorhynchus hendersoni larvae were under 30℃. So, Mass production model of marine ornamental shrimp in accordance with experimental result and depends on the environment to establish standard operating procedure.
4. The ammonia will be found in culture tank, when residual feeds are put to culture water after 30 hours. If the residual feeds were removed by filters, ammonia will not find in culture water. The removal rate of residual feeds in culture water was affected by flow rate of water. The best flow rate of culture water was 12.5 t/hr in this culture system. The ammonia oxidation rate in culture water was increased with the applied voltage increased in photoelectrocatalytic oxidation system. The water quality of flow-through system (FTS) was better than recirculation aquaculture system (RAS) in comparison experiments between RAS and FTS. But the survival rate of Molucca damselfish of two experiments is not remarkably difference. The result was the same to the Atlantic cod (Gadus morhua L.) larvae rearing experiment (Attramadal et al., 2012).
5. Training more than 300 species of fish and Understanding of the food organisms for fry rearing series, larvae demand on water quality and microalgae, to train a large number of reference as seedlings. Observed Acanthuridae feeding and reproduction, adult fish stocking in the temperature control 6 t FRP tank. Small footprint, low-cost equipment and easy maintenance of filtration equipment and egg collection tank, low power consumption. To improve the aquaculture water quality, reduce production costs, Species of fish have been spawning may be a natural pairing, The future can be used as fry production and related facilities planning reference other modules. The Acanthuridae farming technology research and development completed. Including species、juvenile fish cultivation disease prevention.