1、Has completed the investigate to the packaging of ornamental fish, water quality parameters, and survivorship analysis from various supply and source, including the 61 batch and which from 18 country at the least. Heavy pressure, temperature changes, ammonia-N accumulated, without stop to feed before transportation, water quality decline and species-specific bacterial and parasites pathogens were the main factors which makes obvious mortality after long term transportation. Development the techniques of various important species to export trade, including 31 species freshwater and saltwater fish and crustacean. Before transportation can use various anesthetics and effective concentration to reduce the stress of ornamental fish(2-PE(2-phenoxyethanol) 55-75 mg/l、benzocanie 8-65 mg/l、isoeugenol 1.5-4 mg/l、eugenol 2-6 mg/l、clove oil 2-10 mg/l、quinaldine 2.5-6 mg/l). To develop the technology of low-temperature and flowing water stocking (16-22℃), packaging water adjustment, materials selection, water quality control and layered packing, can keep the survivorship above 95% after 96-144 hours simulated transportation, and reduce transport costs by up to 20%, and significantly improved packaging density and value of at least 15%.
2、One Thousand and one hundred newly hatched Hippocampus kuda were reared in two 200-liter PP tanks. The water was filtered to sand filter（20µ）,cartridge（1µ） and UV-light, aerated gently. Newborn seahorses were fed of copepoda immediately which food density was 4-5 inds/㏄ and add more copepoda if the food density too low at noon. The detritus and dead seahorses in the bottom of PP tank should be removed thrice a day in order to keeping the water clean. Dividing the original tank into three tanks which having three hundred and forty juveniles till the 3rd week, now survival rate was 92.72％. The 4th week , dividing three tanks into four tanks again which having two hundred and forty five juveniles, survival rate was 89.09％.and mean body height were 4.1㎝. The food size was increasing with the growth of seahorse during culture period. The 8th week , each 200-liter PP tank had one hundred and fifty seahorses were 7-8㎝ in mean body height. It was the time to be experimentally weaned onto frozen mysids and provided holdfasts for the seahorses to attach. The 12th week, the mean body height of seahorse was 10-12㎝ reaching market size.
3、In the freshwater ornamental fish species, the freshwater stingray are expensive aquarium pets, but ornamental fish breeders generally considered difficult to breed freshwater stingray. The stingray farmers targeted efforts to cultivate the appearance of special lines stingray, so in addition to the specific varieties breeding, cultivation of hybrid individuals is also an important way. The results show that Freshwater stingrays about two years after the birth grow and mature with the ability to reproduce, training bait after feeding artificial diet, may reduce the use of fresh bait.
4、In this study, we obtained 28 cases collected from 7 ornamental fish farms（22 cases from freshwater ornamental fish and 6 cases from seawater ornamental fish）in distinct regions of Taiwan, 2012. Gross external and internal lesions were recorded. Samples were identified using light microscope examination, microbiological examination and polymerase chain reaction (PCR). In examination result, the bacterial disease were 85.7%％ (n=24) in ornamental fish included Vibrio harveyi (n=7), Mycobacterium sp. (n=4) and Aeromonas sp. (n=4) etc. The parasitic disease were 32.1％ (n=9) in ornamental fish included Dactylogyrus sp. (n=4), Trichodina spp. (n=2), Ichthyophthirus sp. (n=1), Oodinium ocellatum (n=1) and Argulus sp. (n=1). The viral disease in ornamental fish included Koi herpesvirus infections (n=3), Iridovirus infections (n=3) and Nodarvirus (n=2). In addition, the multiplex polymerase chain reaction (m-PCR) technique was established to act as a fast and accurate diagnostic method for monitoring Vibrio vulnificus, V. harveyi and V. alginolyticus in ornamental fish. Additionally, we constructed the production medicine information system of Aquatic animals for mobile devices, and will be available to provide services a finish drug inquiry, counseling and treatment services online.
5、Rhynchocinetes uritai is a kind of ornamental shrimps in the special ecological status which may coexist with other ornamental creatures. For the development of artificial culture techniques of R. uritai, different feeding concentrations and starvation were investigated the effect of the growth and survival rate of early larvae. The result showed that, better survival rate and growth were obtained under feeding with the nauplii of Artemia at 0.5-1/ml of feeding on the 5th stage zoea. Survival rate and growth decreased on the zoea which was not fed on the first day after hatching.
6、Acanthuridae family body color bright for the highly economical valuable marine ornamental fishes. Hence, most people caught it harshly from the wild-habitat, which relatively resulting in great damage of coral reef leading to ecology destructive and marine resources depletion. This study was aimed for establishing Blue surgeonfish (Paracanthurus hepatus) and Somber surgeonfish (Zebrasoma flavescens) artificial breeding techniques, monitoring environmental water quality and explore and spawning circumstances of the relationship and to find a suitable food organisms and to spread this technique to farmers as reference and basic information for other fresh water species artificial breeding. The test results show that cultivation system water quality of cultivation system are in compliance with the species of fish, the water temperature in these factors seems obvious related spawning circumstances. Often found, fish health situation will cause damage if Vibrio alginolyticus was the dominant species.Water quality monitoring for early detection of changes in the culture environment, easy early treatment to improve in order to reduce casualties. As food organisms to protozoan test currently foster four biological results are less than ideal, and more difficulties need to continue to overcome, In this case, living feed is crucial for larvae first feeding, in order to increase the quantities of both species.
7、In recent years, the numbers of marine aquarium fish trade has increased. Damselfish are the very important species in the ornamental fish trade and most are captured from the field. The objective of this study was to establish the breeding and reproductive technologies of damselfish and to reduce the trading negative repercussions on marine ecosystems. In this year, we bred 14 damselfish species and cultured 27,000 fish fry. The fertilized eggs of Chrysiptera parasema were transparent and ellipsoidal, and were 1.21±0.05 mm in total length and 0.55±0.02 mm in width. The yolks were 0.56±0.17 mm long and contained several oil droplets. The radius of the maximum oil droplets are 0.10±0.01 mm. The newly hatched larvae were 2.40±0.23 mm in length. There was no obvious difference among the body weight and body length of male and female fish.