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性類固醇激素對海鱺性別分化之效果

  • 出版日期:111-06-30
  • 標題title(英):
    Effects of Sex Steroids on Gonadal Differentiation in Cobia, Rachycentron canadum
  • 作者:李彥宏‧林承澤‧黃維能‧高鈺勛‧周楷倫
  • 作者auther(英):Yen-Hung Lee, Cheng-Tse Lin, Wei-Neng Huang, Yu-Syun Gao and Kai-Lun Chou
  • 卷別:30
  • 期別:1
  • 頁碼:37-44

魚類單一性別養殖對產業有極大幫助,而海鱺 (Rachycentron canadum) 是臺灣重要的箱網養殖魚種,研究其性別控制,期望能養殖全雌或全雄性海鱺,增加養殖效益及產業利益。本研究是以不同劑量的雌二醇 (17β-estradiol, E2) 及甲基睪固酮激素 (Methyltestosterone, MT) 添加在飼料中,對海鱺仔魚進行性別調控試驗,以了解海鱺生殖腺性別分化時間以及控制海鱺性別激素的有效濃度。經觀察海鱺生殖腺組織切片結果顯示,仔魚性別分化時期約在孵化後 (days post hatching, dph) 50–70天之間。餵食5 mg E2/kg飼料組,95.6% 仔魚在120 dph為雌性;50 mg E2/kg飼料組,則於99 dph達到100% 雌性化效果,但高濃度E2也會造成幼魚成長緩慢。另外,餵食45 dph幼魚MT飼料55天 (100 dph),25 mg與75 mg MT/kg兩組之海鱺,雄性比例均為100%,有完全雄性化效果,但也會導致幼魚成長緩慢。總之,餵食添加性類固醇激素之飼料確實能控制海鱺性別,可應用在海鱺種苗培育過程,達到生產全雌化或全雄化子代,有助於海鱺養殖產業發展。

摘要abstract(英)


Monosex, all female or all male progenies, is important in fish production to increase the benefits to the aquaculture industry. Cobia (Rachycentron canadum) is a potential candidate for sea-cage culture in Taiwan. In this study, the sex steroids estradiol (E2) and methyltestosterone (MT) were added at various doses to the feed of cobia to control its sex. Gonadal sex differentiation of cobia larvae was apparent 50-70 days post-hatching (dph). Gonadal differentiation was promoted by the administration of sex steroids. The feminization rate of cobia fed a commercial feed with E2 added at doses of 5 and 50 mg kg-1 was 95% and 100%, respectively; the feeding periods were 55-120 dph and 55-99 dph for the low- and high-dose treatments, respectively. The masculinization rate of cobia given feed with MT added at doses of 25 and 75 mg kg-1 was 100% in both cases for a feeding period of 45-100 dph. In conclusion, our results show that the sex of cobia can be controlled by exogenous sex steroids and our protocol can be used in the breeding and reproduction process of cultured cobia