農業部-水產試驗所全球資訊網農業部-水產試驗所全球資訊網

為節省九孔稚貝剝離附著板之人力，嚐試利用Tricaine methanesulfonate（MS-222）和Ethyleneglycol monophenyl ether（2-PE）二種麻醉劑針對九孔稚貝剝離進行試驗，以九孔稚貝殼長在4.2-5.9 mm為材料，在不同範圍之試驗濃度（MS-222, 50-350 ppm; 2-PE, 200-700 ppm）下，其剝離率、蘇醒率及七天內之活存率皆達100%，但以刷子剝離之對照組，其一天及七天內之活存率分別為 95% 及85%，麻醉劑顯然有較高之活存率。二種麻醉劑剝離所需時間隨麻醉劑濃度的升高而縮短，而蘇醒時間則隨麻醉劑濃度的升高而增加。為符合繁殖業者之需要，即剝離率及活存率均在 100，而且，剝離與蘇醒時間最短之要求下，MS-222濃度在 75-250 ppm，其剝離及蘇醒時間相加所需時間在 20 分鐘內，而2-PE濃度在200-300 ppm， 所需時間也在 20 分鐘，故此上述濃度可做為九孔稚貝剝離之用，以節省人力之浪費。
關鍵詞：麻醉劑，九孔，剝離

This experiment aimed to determine the feasibility of saving manpower by using tricanine methanesulfonate (MS-222, 50-350 ppm) and ehyleneglycol monophenyl ether（2-PE, 200-700 ppm）on exfoliation for juvenile abalone Haliotis diversicolor supertexta, shell length of 4.2-5.9 mm. The results revealed that the exfoliation rate, the recovery rate, and the one-week survival rate of the animals were reached to 100%. The above-mentioned anesthetics gave higher survival rate as compared to the traditional brush method on exfoliation for juvenile abalone of which their survival rate in one-day and one-week period were 95% and 85%, respectively. Time for exfoliation and time for recovery was inversely and linearly related to the anesthetics concentration, respectively. To meet the demand of a 100% exfoliation and survival rate and the least time of exfoliation and recovery for abalone hatchery farmers, we suggested that the following safety concentration levels: 175-250 ppm of MS-222 and 200-300 ppm of 2-PE.
Key words: Anesthetics, Abalone, Exfoliation