1. In order to understand practicablity cryopreserved sperm of small abalone (Haliotis diversicolor )sperm, which have established in 2016, using this sperm that has been cryopreserved for 180 days and one day under the same thawing conditions fertilized test at concentration of 10 2 / mL to 10 3 / mL at 23-25 ° C, comparing the occurrence of embryos in two cell stages, as a reference for evaluating the quality of sperm liquid nitrogen storage. The results showed that the fertilized rate was 4.07±4.09% and 20.74±5.48%. In addition, the use of published Abalone shriveling syndrome associated virus (AbSV) virus-related genes as genetic markers of sperm screening to exclude suspected pathogen-infected samples.
2. In study the cryopreservation of giant grouper ( Epinephelus lanceolatus ) sperm , mature E. lanceolatussemen was used as experimental material, and concentration of various semen diluents, and concentration of cryoprotectant were screened. We used liquid nitrogen and 2.0 ml cryovials to preserve sperms by step cooling procedure. The result showed the cryopreservation of E. lanceolatus semen that Hank’s solution and Marine Fish Ringeras semen diluents was better than MPRS solution as semen duluents, which preserved 90.33% ±0.58% and 91.00% ±1.00% sperm motility after unfrozen. Using Hank’s solution as the liquid foundation to prepare cryoprotectant, ,no significant difference was observed in sperm motility by 12% and 18% DMSO cryopreservation (P>0.05) . The best of all is 12% DMSO, which preserved 74.61% ± 4.57 % sperm motility respectively after unfrozen. Using cryopreserved E. lanceolatus semen to fertilize E. moara eggs, the rates of fertilization were 90.37 % ± 8.71% , without difference with fresh sperms. This study showed that 12% DMSO using Hank’s solution as the liquid foundation could cryopreserve E. lanceolatus semen .We established a frozen sperm library based on this study, and it will provide a basis for the artificial breeding and cross breeding.
3. The purpose of this program is to provide the tilapia industry with new techniques that were developed by the Germplasm Bank of Freshwater Fish, Fisheries Research Institute. These techniques will be promoted to the industry with following goals to enhance the competitive strength.(1)The technology transfer of genetically male tilapia.(2)The supply of 500,000 fries of Nile tilapia with the feature of high growth rate.(3)The application of molecular protocols to analysis the reason of low male-ratio of tilapia fry. Although the FRI could provide the aquaculture industry with a high-growth tilapia to commercialization but could not match and through licensing agreements in this year. In addition, we try to analyze the sex-determining markers of genetically female and try to realize the characters. The results show that breeding on the genetic female still cannot breed high female-ratio stably. Otherwise, the genetic female marker could be applied on body development in the future. 4.20 mature giant clamsTridacna maxima were collected for artificial propagations. More than 10 thousands of juveniles were produced in three batches and 5000 of them reached >1 cm in shell length until the end of 2017.Spawning can be successfully induced by dry-out treatment combined with temperature fluctuation application.Fertilized eggs metamorphosed into settled juveniles with water conditions under 28℃ and 33psu within 14-15 days.The grow-out cage was established for broodstock and juveniles to escape from mass mortalities caused by chill event during winter time.