農業部-水產試驗所全球資訊網農業部-水產試驗所全球資訊網

快速增長是一個重要高度期望之性狀，從而影響物種生產盈利能力，相對於產業會 有重大影響，從另一角度思考，提高飼料利用率減少成本支出及維持好的養殖用水 是兼容並顧的，因此成功選擇最佳生長速率或體重性狀，是水產育種計畫的一個關 鍵目標。本研究利用傳統育種表現型的篩選，並配合微衛星標記追蹤表現型與基因 型之間關係，利用數量遺傳學探討量化性狀及影響因素、測量值與相互關係，試驗 方法為追蹤與骨骼肌生成相關基因之微衛星篩選分析，進行其中一組DNA缺失分子標 誌與體重差異追蹤試驗，觀測結果進行one way ANOVA分析發現，兩族群中具有缺失 型標誌的魚隻個體體重都有顯著較輕，並有顯著差異，DT1族群P值為0.035；DT2族 群P值達極顯著水準為0.003；DT3族群P值達極顯著水準為0.012確實證明具有此缺失 型DNA SSR為生長較緩慢表現型，可應用於未來育種相關試驗及魚隻選育手段。另外 基因型與體表性狀統計，利用微衛星 DNA 標誌來分析基因型與體重的相關性，共有 DT1、DT2、DT3不同試驗族群中，DT1與DT3各有 9 個和DT2 6 個分子標誌在體重和 基因型相關性達到顯著水準，在三族群中同時達到顯著水準共有 5 個微衛星標誌 (Ela8112d Ela348、Ela9156b、Ela8112a、Ela22361)顯示龍膽石斑多型性微衛星有 潛力做為分子標誌以進行分子標誌輔助育種 (MAS)，未來可應用於建立分子標誌輔 助育種平台。

Successful selection for optimal growthrate or body weight is a key objective in aquaculture breeding programs.Traditional phenotype-based selectionis typically used to select for growth traits, however, it requires severalgenerations to optimize genetic improvement. In addition, insight into thegenetic bases of growth can be used to make better selection decisions.Marketdemand for the orange-spotted grouper has prompted the development of shfamilies and populations characterized by lower food consumption and highergrowth rates.Test Method for Tracking and skeletalmuscle first generation micro satelliterelated gene screening analysis, thefinal Phenotype and genotype statistics, using 11 microsatellite DNA markers toanalyze the correlation between genotype and body weight, DT1 and DT3 indifferent ethnic groups each nine and DT2 six molecular markers correlatedreached significant level in body weight and genotype in the Three groups atthe same time achieve significant level there are five microsatellite markers(Ela8112d, Ela348, Ela9156b, Ela8112a, Ela22361) show gentian grouperpolymorphic microsatellite molecular markers have the potential to be used asmolecular markers for assisted breeding (MAS), the future can be used to establishmolecular markers assisted breeding platform.