In this study, there are three batches of fourfinger threadfin (Eleutheronema tetradactylum), the first batch is a 3-year-old fish, which comes from the offspring of the fish bred by fertilized eggs introduced from Singapore, and the second batch (2-year-old fish) comes from the fry bred by fishermen, with about 400 fish. The third batch is the offspring of 3-year-old fish this year, with about 1,200 fish. The hatching rate of fry is 92%, and more than 30,000 larvae can be cultivated, with a breeding rate of 33%. Fish spawn 33 times, and the spawning temperature is 26.7~29℃. The study raised the effect of different salinities (10, 20, 25, 30 psu) on fourfinger threadfin stock, and holding to solve problems of aquaculture on the fish under global climate change. We record weight gain rate(WGR), feed conversion ratio(FCR), and survival rate(SR) during stock, and the concentration of cortisol and melatonin in plasm. The results show that the fish in 10psu during 90 days stock present best WGR (211.2± 18.3%) and best FCR (1.4± 0.2), and fish in 20psu present best SR (88.3± 1.4%). In addition, the fish in 20psu during 30 days stock present highest plasma cortisol (21.5± 4.1 ng/ml), and appear the lowest concentration in 30psu (9.8± 1.2ng/ml), that the cortisol decrease trend with increasing salinity. In contrast, plasma melatonin shows an upward trend with increasing salinity in the fish, that present the highest (3.3± 0.1ng/ml) in 25psu and the lowest (1.2± 0.3ng/ml) in 10psu. The establishment of basic data under different salinity stocking of Eleutheronema tetradactylum can continue to be used for future research applications.