Nervous necrosis virus (NNV) infective grouper larvae that cause high mortality, resulting in serious economic losses in aquaculture industry.The final aim of this project applies proteomic method to analyze viral protein and virus-host interaction through different expressed proteins after virus infection. It will provide knowledge of biological processes of nervous necrosis virus. The second year of this project uses grouper cell line which high susceptive with NNV to perform proteomic analysis.It will establish 2-DE protein profiles after NNV infection by twodimensional
gel electrophoresis, in-gel digestion, MS sequencing and
protein database analysis.