農業部-水產試驗所全球資訊網農業部-水產試驗所全球資訊網

本計畫之目的在於建立SPF白蝦種蝦庫並選育優良白蝦品系。所得結果如下：1.白蝦種原之引進：目前已自美國引進並保存SPF白蝦種原5批。2.病原追蹤檢測：持續以PCR及qPCR檢測技術篩選不帶WSSV、TSV、GAV/MBV、IMNV及IHHNV等6種病毒及EMS的病原菌-副溶血弧菌的白蝦種原，並參加水產動物疾病監測計畫，結果連續2年未曾檢出。3.高成長之選育：本試驗使用上年度高生殖力母蝦的子代15批，進行三階段之高成長選育。第一階段篩選強度為26.2%，篩選比例為1.7，第二階段篩選強度為27.8%，公蝦篩選比例為1.11，母蝦為1.21，第三階段篩選強度約為25%，合計篩選強度約為1.8%。4.高生殖力之選育：本試驗共使用100尾母蝦，在為期10個月的記錄中，個別產卵次數的差異很大，其中有15尾產卵次數是0，而產卵數12次以上者共計14尾，佔100尾母蝦的14%，共產卵191次佔總產卵559次的34%。

The objectives of the project are to establish the SPF broodstock of Litopenaeus vannamei and to improve high growth and high reproductive performance through selective breeding. The results are following: 1. Introduce of broodstock: Five batches of broodstock were introduced and kept in Tungkang Biotechnology Research Center, Taiwan Fisheries Research Institute. 2. Diseases diagnose: broodstock were screened with WSSV, TSV, YHV/GAV, IMNV and IHHNV using qPCR and EMS pathogen, Vibrio parahaemolyticus. WSSV, TSV, YHV and IHHNV were also not detected for 2 years by teams of the aquatic animal disease surveillance program. 3. selection for high growth: This study using the offspring of 15 females with high reproductive performance to go through 3-step high growth selection program. The selective intensity for the first, the second and the third step of selection were 26.2%, 27.8% and 25.0%. 4. selection for high reproductive performance: the number of spawn were highly variable within 100 females used in this study, in which, 15 no spawn at all; 14 spawn more than 12 times, which spawn 191 times in total and represents 34% of the grand total of 559 times.